| Cast list | | Spencer Tracy | as | Dr. Henry Jekyll/Mr. Hyde | | Ingrid Bergman | as | Ivy Peterson | | Lana Turner | as | Beatrix Emery | | Donald Crisp | as | Sir Charles Emery | | Ian Hunter | as | Dr. John Lanyon | | Barton MacLane | as | Sam Higgins | | C. Aubrey Smith | as | Bishop Manners | | Peter Godfrey | as | Poole, Jekyll's Butler | | Sara Allgood | as | Mrs. Higgins | | Frederick Worlock | as | Dr. Heath (as Frederic Worlock) | | William Tannen | as | Intern Fenwick | | Frances Robinson | as | Marcia | | Denis Green | as | Freddie | | Billy Bevan | as | Mr. Weller | | Forrester Harvey | as | Old Prouty |
| | Crew list: | | Victor Fleming (Director) | | Robert Louis Stevenson (Writer) | | John Lee Mahin (Writer) |
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