| Cast list |
| Maxwell Atoms | as | Jeff the Spider (voice) |
| Diedrich Bader | as | Hoss Delgado / Fiskerton (voice) |
| Dee Bradley Baker | as | Numbuh 4 / Toilenator / Echo Echo (voice) |
| Greg Baldwin | as | Aku (voice) |
| Kathleen Barr | as | Marie Kanker (voice) |
| Jeff Bennett | as | Johnny Bravo / Ace (voice) |
| Steve Blum | as | Vilgax / TOM (voice) |
| Corey Burton | as | V.V. Argost (voice) |
| Cathy Cavadini | as | Blossom (voice) |
| Greg Cipes | as | Kevin Levin (voice) |
| Kathryn Cressida | as | Dee Dee (voice) |
| Jim Cummings | as | Fuzzy Lumpkins (voice) |
| Elizabeth Daily | as | Buttercup (voice) |
| Eddie Deezen | as | Mandark (voice) |
| Grey DeLisle | as | Frances 'Frankie' Foster / Mandy (voice) |
| David DeLuise | as | Coop (voice) |
| John Di Maggio | as | The Scotsman / Schnitzel (voice) |
| Ben Diskin | as | Numbuh 1 / Numbuh 2 (voice) |
| Greg Eagles | as | Grim (voice) |
| Paul Eiding | as | Grandpa Max (voice) |
| Dave Fennoy | as | Tetrax Shard (voice) |
| Keith Ferguson | as | Blooregard Q. Kazoo (voice) |
| Jenn Forgie | as | May Kanker (voice) |
| Marty Grabstein | as | Courage (voice) |
| C.H. Greenblatt | as | Fred Fredburger (voice) |
| Jennifer Hale | as | Princess Morbucks (voice) |
| Mark Hamill | as | Stickybeard (voice) |
| Matt Hill | as | Ed (voice) |
| Richard Steven Horvitz | as | Billy (voice) |
| Roger Jackson | as | Mojo Jojo (voice) |
| Janyse Jaud | as | Lee Kanker (voice) |
| Ashley Johnson | as | Gwen Tennyson (voice) |
| Nicky Jones | as | Chowder (voice) |
| Tom Kane | as | Professor Utonium /Him / Mr. Herriman (voice) |
| Tom Kenny | as | Eduardo / Mayor (voice) |
| Maurice LaMarche | as | Father (voice) |
| Phil LaMarr | as | Samurai Jack / Wilt / Dracula (voice) |
| Sam Lerner | as | Zak Saturday (voice) |
| Yuri Lowenthal | as | Ben Tennyson / Albedo (voice) |
| Sean Marquette | as | Mac (voice) |
| Richard McGonagle | as | Four Arms (voice) |
| Lara Jill Miller | as | Juniper Lee (voice) |
| Candi Milo | as | Dexter / Coco / Cheese (voice) |
| Phil Morris | as | Doc Saturday (voice) |
| Rob Paulsen | as | Major Glory (voice) |
| Khary Payton | as | Hex (voice) |
| Kevin Michael Richardson | as | Demongo (voice) |
| Tony Sampson | as | Eddy (voice) |
| Dwight Schultz | as | Thromnambular (voice) |
| Kath Soucie | as | Computress (voice) |
| Tara Strong | as | Bubbles (voice) |
| Nicole Sullivan | as | Drew Saturday (voice) |
| Cree Summer | as | Numbuh 5 (voice) |
| Lauren Tom | as | Numbuh 3 (voice) |
| Thurop Van Orman | as | Flapjack (voice) |
| Sam Vincent | as | Double D (voice) |
