| Cast list |
| Edward Norton | as | Bruce Banner (voice) |
| Liv Tyler | as | Betty Ross (voice) |
| Tim Roth | as | Emil Blonsky (voice) |
| William Hurt | as | Gen. Thaddeus 'Thunderbolt' Ross (voice) |
| Tim Blake Nelson | as | Samuel Sterns (voice) |
| S. Scott Bullock | as | Vector /Jupiter Leader /Hulkbuster (voice) |
| Jon Curry | as | Rick Jones /Abomination (voice) |
| Chris Edgerly | as | Enclave Soldier / Stark Industries Hulkbuster /Bi-Beast's Bottom Head (voice) |
| Keith Ferguson | as | X-Ray / Army Soldier /Bi-Beast's Top Head (voice) |
| Simbi Khali | as | Ceres Leader (voice) (as Simbi Khali Williams) |
| Mitch Lewis | as | Ironclad / Cop / Male Computer (voice) |
| Rachael MacFarlane | as | Vapor / Female Pedestrian (voice) |
| Jeffrey Parker | as | Abomination Vocal Effects (voice) |
| Stephen Stanton | as | Iron Man / Male Pedestrian #2 (voice) |
| Fred Tatasciore | as | Hulk (voice) |
| Courtenay Taylor | as | Summervox /Minerva Leader (voice) |
| Dave Wittenberg | as | Male Pedestrian #1 / Special Forces Leader / Vulcan Leader (voice) |
