| Cast list |
| Joe Buck | as | Himself - Play-by-Play Announcer |
| Tim McCarver | as | Himself - Color Commentator |
| Ken Rosenthal | as | Himself - Field Reporter |
| Jose Mota | as | Himself - Field Reporter |
| Eric Byrnes | as | Himself - Field Reporter |
| Jeanne Zelasko | as | Herself - Pregame Host |
| Kevin Kennedy | as | Himself - Pregame Analyst |
| Eric Karros | as | Himself - Pregame Analyst |
| Josh Beckett | as | Himself - AL Pitcher, Boston Red Sox |
| Carlos Beltran | as | Himself - NL Starting Center Fielder, New York Mets |
| Bruce Bochy | as | Himself - NL Coach, San Francisco Giants |
| Barry Bonds | as | Himself - NL Starting Left Fielder, San Francisco Giants |
| Miguel Cabrera | as | Himself - NL Third Baseman, Florida Marlins |
| Francisco Cordero | as | Himself - NL Relief Pitcher, Milwaukee Brewers |
| Carl Crawford | as | Himself - AL Left Fielder, Tampa Bay Devil Rays |
| Prince Fielder | as | Himself - NL Starting First Baseman, Milwaukee Brewers |
| Brian Fuentes | as | Himself - NL Relief Pitcher, Colorado Rockies |
| Ron Gardenhire | as | Himself - AL Coach, Minnesota Twins |
| Ken Griffey Jr. | as | Himself - NL Starting Right Fielder, Cincinnati Reds |
| Vladimir Guerrero | as | Himself - AL Starting Right Fielder, Los Angeles Angels of Anaheim |
| Carlos Guillen | as | Himself - AL Shortstop - Detroit Tigers |
| Cole Hamels | as | Himself - NL Pitcher, Philadelphia Phillies |
| J.J. Hardy | as | Himself - NL Shortstop, Milwaukee Brewers |
| Danny Haren | as | Himself - AL Starting Pitcher, Oakland Athletics |
| Trevor Hoffman | as | Himself - NL Relief Pitcher, San Diego Padres |
| Matt Holliday | as | Himself - NL Left Fielder, Colorado Rockies |
| Orlando Hudson | as | Himself - NL Second Baseman, Arizona Diamondbacks |
| Torii Hunter | as | Himself - AL Center Fielder, Minnesota Twins |
| Chris Isaak | as | Himself - National Anthem performer |
| Bobby Jenks | as | Himself - AL Relief Pitcher, Chicago White Sox |
| Derek Jeter | as | Himself - AL Starting Shortstop, New York Yankees |
| Kenney Dale Johnson | as | Himself - National Anthem performer |
| Al Kaline | as | Himself - AL Honorary Captain |
| John Lackey | as | Himself - AL Pitcher, Los Angeles Angels of Anaheim |
| Tony LaRussa | as | Himself - NL Manager, St. Louis Cardinals |
| Carlos Lee | as | Himself - NL Left Fielder, Milwaukee Brewers |
| Derrek Lee | as | Himself - NL First Baseman, Chicago Cubs |
| Jim Leyland | as | Himself - AL Manager, Detroit Tigers |
| Mike Lowell | as | Himself - AL Third Baseman, Boston Red Sox |
| Victor Martinez | as | Himself - AL Catcher, Cleveland Indians |
| Russell Martin | as | Himself - NL Starting Catcher, Los Angeles Dodgers |
| Willie Mays | as | Himself - Ceremonial 1st Pitch / NL Honorary Captain |
| Brian McCann | as | Himself - NL Catcher, Atlanta Braves |
| Gil Meche | as | Himself - AL Pitcher, Kansas City Royals |
| Justin Morneau | as | Himself - AL First Baseman, Minnesota Twins |
| Hideki Okajima | as | Himself - AL Relief Pitcher, Boston Red Sox |
| Magglio Ordóñez | as | Himself - AL Starting Left Fielder, Detroit Tigers |
| David Ortiz | as | Himself - AL Starting First Baseman, Boston Red Sox |
| Roy Oswalt | as | Himself - NL Pitcher, Houston Astros |
| Jonathan Papelbon | as | Himself - AL Relief Pitcher, Boston Red Sox |
| Jake Peavy | as | Himself - NL Starting Pitcher, San Diego Padres |
| Bradley Wayne Penny | as | Himself - NL Pitcher, Los Angeles Dodgers |
| Placido Polanco | as | Himself - AL Starting Second Baseman, Detroit Tigers |
| Jorge Posada | as | Himself - AL Catcher, New York Yankees |
| Albert Pujols | as | Himself - NL First Baseman, St. Louis Cardinals |
| J.J. Putz | as | Himself - AL Relief Pitcher, Seattle Mariners |
| Manny Ramirez | as | Himself - AL Left Fielder, Boston Red Sox |
| Willie Randolph | as | Himself - NL Coach, New York Mets |
| Jose Reyes | as | Himself - NL Starting Shortstop, New York Mets |
| Alex Rios | as | Himself - AL Left Fielder, Toronto Blue Jays |
| Brian Roberts | as | Himself - AL Second Baseman, Baltimore Orioles |
| Alex Rodríguez | as | Himself - AL Starting Third Baseman, New York Yankees |
| Francisco Rodríguez | as | Himself - AL Relief Pitcher, Los Angeles Angels of Anaheim |
| Ivan Rodriguez | as | Himself - AL Starting Catcher, Detroit Tigers |
| Aaron Rowand | as | Himself - NL Center Fielder, Philadelphia Phillies |
| C.C. Sabathia | as | Himself - AL Pitcher, Cleveland Indians |
| Takashi Saitô | as | Himself - NL Relief Pitcher, Los Angeles Dodgers |
| Freddy Sanchez | as | Himself - NL Second Baseman, Pittsburgh Pirates |
| Johan Santana | as | Himself - AL Pitcher, Minnesota Twins |
| Ben Sheets | as | Himself - NL Pitcher, Milwaukee Brewers |
| Grady Sizemore | as | Himself - AL Center Fielder, Cleveland Indians |
| John Smoltz | as | Himself - NL Relief Pitcher, Atlanta Braves |
| Alfonso Soriano | as | Himself - NL Left Fielder, Washington Nationals |
| Ichiro Suzuki | as | Himself - AL Starting Center Fielder, Seattle Mariners |
| Chase Utley | as | Himself - NL Starting Second Baseman, Philadelphia Phillies |
| Jose Valverde | as | Himself - NL Relief Pitcher, Arizona Diamondbacks |
| Justin Verlander | as | Himself - AL Pitcher, Detroit Tigers |
| Billy Wagner | as | Himself - NL Relief Pitcher, New York Mets |
| Ron Washington | as | Himself - AL Coach, Texas Rangers |
| Brandon Webb | as | Himself - NL Pitcher, Arizona Diamondbacks |
| David Wright | as | Himself - NL Starting Third Baseman, New York Mets |
| Chris Young | as | Himself - NL Pitcher, San Diego Padres |
| Dmitri Young | as | Himself - NL First Baseman, Washington Nationals |
| Michael Young | as | Himself - AL Shortstop - Texas Rangers |
