| Cast list |
| Josh Flitter | as | Ace Ventura Jr |
| Emma Lockhart | as | Laura |
| Austin Rogers | as | A-Plus |
| Chris Adler | as | Pennington Jr's Body Guard |
| Robie Alan | as | Special Government Agent |
| Reed Alexander | as | Quenton Pennington Jr |
| Christian Andreas | as | Guard in dinosaur chase |
| Karl Anthony | as | Laura's Dad |
| Sam Bauso | as | FBI Agent |
| Jessica Blackmore | as | Assistant with white horse |
| Justin Brown | as | Churro Guy |
| Jeff Chase | as | Penningtons Guard |
| Brian Patrick Clarke | as | Pennington |
| Jim R. Coleman | as | Judge Barnes |
| John Connon | as | Tour Guide |
| Whitney Costner | as | Witness |
| Ann Cusack | as | Melissa Ventura |
| Cullen Douglas | as | Dr. Sickinger |
| Tori Eadie | as | Little Laura with pony |
| Omar Elkalyoubie | as | Chinese Dignitary |
| Bayley Ellenburg | as | Witness |
| Jeff Ellenburg | as | Wittness |
| Emily Rose Everhard | as | Additional voices (voice) |
| Kendall Ganey | as | Little Girl - Spelling Bee |
| Ciara Gari | as | Student |
| Wael Georgey | as | FBI Agent |
| Lisa Glaze | as | Director |
| Brandi Hanson | as | Tara's friend |
| William Haze | as | Magician |
| Tom Hillmann | as | The Trooper |
| Kelsie Erin Howell | as | Party Kid |
| Ariana Kollman | as | Spelling Bee Girl |
| Art LaFleur | as | Russell Hollander |
| Trevin Lica | as | Bully #1 |
| Leslie Maine | as | FBI Agent |
| Amari Marshall | as | Student |
| Tiffany Marshall | as | Crash Driver / Cafeteria Worker |
| Angel McBride | as | iPod Gir - Tara's Friend |
| Celestial McBride | as | Spelling Bee Contestant - Marina's Friend |
| Melody Mccraney | as | Teacher |
| Ashley Milchman | as | Emo Kid #1 |
| Brooke Milchman | as | Emo Kid #2 |
| Jennifer Paige Moralez | as | Student / Party Dancer |
| Tom Myers | as | Security Guard |
| Jeremy Palko | as | CSI Agent |
| Aubrey Peeples | as | Daniella |
| Jason Nathaniel Powers | as | Park Guest |
| Michael Rummel | as | Basketball / Wrestling Referee |
| David W. Scott | as | Mr. Pennington's Body Guard |
| AJ Semiday | as | Teacher |
| Adonis Stevens | as | DJ the Security Guard |
| Andy Ussach | as | Pennington's Butler |
| Aries Marvin Uzzle | as | FBI Agent |
| Nelitza Vallellanes | as | Teacher |
| Adam Vernier | as | MIB Agent |
| Ralph Waite | as | Grandpa Ventura |
| Wolfgang J. Weber | as | Crash Driver |
