| Cast list |
| Will Friedle | as | Terence 'Terry' McGinnis/Batman (voice) |
| Kevin Conroy | as | Bruce Wayne/Batman (voice) |
| Mark Hamill | as | Jack Napier/The Joker, Jordan Price (voice) |
| Angie Harmon | as | Commissioner Barbara Gordon (voice) |
| Dean Stockwell | as | Communications Engineer Mr. Timothy 'Tim' Drake (voice) |
| Teri Garr | as | Mrs. Mary McGinnis (voice) |
| Arleen Sorkin | as | Dr. Harleen Quinzel/Harley Quinn/'Nana' Harley (voice) |
| Tara Strong | as | Barbara Gordon/Batgirl (voice) |
| Mathew Valencia | as | Timothy 'Tim' Drake/Robin (voice) |
| Melissa Joan Hart | as | Delia & Deidre Dennis/Dee Dee (voice) |
| Don Harvey | as | Charles Buntz/Chucko (voice) |
| Michael Rosenbaum | as | Stewart Carter Winthrop III/Ghoul (voice) |
| Frank Welker | as | Woof the Hyena-Man, Ace the Bathound (voice) |
| Henry Rollins | as | Benjamin 'Ben' Knox/Bonk (voice) |
| Rachael Leigh Cook | as | Chelsea (voice) |
