| Cast list |
| William Petersen | as | Gil Grissom (voice) (as William L. Petersen) |
| Edie Mirman | as | Catherine Willows (voice) |
| Kate Savage | as | Sara Sidle (voice) |
| Gary Dourdan | as | Warrick Brown (voice) |
| George Eads | as | Nick Stokes (voice) |
| Paul Guilfoyle | as | LVPD Capt. Jim Brass (voice) |
| Robert David Hall | as | Dr. Al Robbins (voice) |
| Eric Szmanda | as | Greg Sanders (voice) |
| Sally Clawson | as | Carla Mitchell / Carrie Canelli / Narrator (voice) |
| Andrew Chaikin | as | Andy Penmore / Michael Dubois (voice) |
| David W. Collins | as | Craig Landers (voice) |
| Jarion Monroe | as | Nathan Ackerman (voice) |
| Sumalee Montano | as | Lucy Canelli / Maya Nguyen (voice) |
| Sydney Rainin | as | Emily Hanson / Gun Club Clerk (voice) |
| Bob Sarlotte | as | Lou Astor / Patrick Milton / Gus Clein (voice) |
