| Cast list |
| Dee Bradley Baker | as | Nightcrawler (voice) |
| Adrienne Barbeau | as | Sif (voice) |
| Gregg Berger | as | Attuma /Galactus /Thing (voice) |
| Steve Blum | as | Wolverine /Venom (voice) (as Steven Jay Blum) |
| Trev Broudy | as | Captain America (voice) |
| Gabrielle Carteris | as | Elektra /Enchantress (voice) |
| Larry Cedar | as | Loki (voice) |
| Cam Clarke | as | Thor /Daredevil /Weasel (voice) |
| Chris Cox | as | Silver Surfer (voice) |
| John Cygan | as | Iron Man (voice) |
| Robin Atkin Downes | as | Cyclops /Dr. Bruce Banner /Crimson Dynamo (voice) |
| Greg Eagles | as | Luke Cage (voice) (as Gregg Eagles) |
| Paul Eiding | as | Ymir (voice) |
| Quinton Flynn | as | Spider-Man /Arcade (voice) |
| Crispin Freeman | as | Winter Soldier (voice) |
| Nika Futterman | as | Black Widow /Deathbird (voice) (as Nikka Futterman) |
| Michael Gough | as | MODOK (voice) |
| Richard Green | as | Magneto (voice) |
| Kim Mai Guest | as | Crystal (voice) |
| Michael Hagiwara | as | Wong (voice) |
| Arin Hanson | as | Dr. Bruce Banner (voice) |
| Dan Hay | as | Additional Voices (voice) |
| James Horan | as | Doctor Strange /Ultron (voice) |
| Jerry Houser | as | Hank Pym (voice) |
| Marabina Jaimes | as | Clea / Ronin (voice) |
| Tom Kane | as | Grey Gargoyle / Kurse /Professor Xavier (voice) |
| John Kassir | as | Deadpool (voice) |
| Josh Keaton | as | Human Torch (voice) |
| Phil LaMarr | as | Black Panther /Colossus /Moon Knight /Uatu (voice) (as Phil Lamarr) |
| Travis Landry | as | Additional Voices (voice) |
| Dawnn Lewis | as | Storm (voice) |
| Nancy Linari | as | Medusa (voice) |
| Peter Lurie | as | Executioner / Hulk /Sabretooth (voice) |
| Scott MacDonald | as | Announcer / Corsair /Cyclops /Dum-Dum Dugan /Nick Fury (voice) |
| Danica McKellar | as | Invisible Woman (voice) |
| David Naughton | as | Mister Fantastic (voice) |
| Nolan North | as | Ghost Rider /Hawkeye (voice) |
| Khary Payton | as | Blade (voice) |
| Phil Proctor | as | Baron Mordo /Edwin Jarvis (voice) |
| Peter Renaday | as | Odin (voice) |
| Clive Revill | as | Dr. Doom (voice) |
| Roger Rose | as | Vision /Captain Marvel (voice) |
| James Sie | as | Ancient One /Fin Fang Foom /Mandarin /Radioactive Man (voice) |
| David Sobolov | as | Blackheart / Titannus (voice) |
| April Stewart | as | Ms. Marvel (voice) |
| Fred Tatasciore | as | Mephisto (voice) |
| James Arnold Taylor | as | Iceman (voice) (as James Taylor) |
| Joe Thomas | as | Namor the Sub-Mariner (voice) (as Joe J. Thomas) |
| Tasia Valenza | as | Spider-Woman (voice) (as Tasia Vallenza) |
| Sarah Waits | as | Jean Grey (voice) |
| Beau Weaver | as | Scorpion / Tiger Shark (voice) |
| Dave Wittenberg | as | Gladiator (voice) |
| Kris Zimmerman | as | Additional Voices (voice) |
