| Cast list |
| William Petersen | as | CSI Head Investigator Gilbert 'Gil' Grissom (voice) |
| Marg Helgenberger | as | CSI Level 3 Catherine Willows (voice) |
| Gary Dourdan | as | CSI Level 3 Warrick Brown (voice) |
| George Eads | as | CSI Level 3 Nick Stokes (voice) |
| Jorja Fox | as | Sara Sidle (voice) |
| Robert David Hall | as | Chief Medical Examiner Dr. Albert 'Al' Robbins (voice) |
| Paul Guilfoyle | as | LVPD Capt. Jim Brass (voice) |
| Eric Szmanda | as | Lab Technician Greg Sanders (voice) |
| Jodi Regts | as | Narrator (voice) |
| Jason Nicoloff | as | Ace Dillinger (voice) (as Jason Nickolaff) |
| Jason Simpson | as | David McLadden (voice) |
| Louis Chirillo | as | Cory Muzotti (voice) |
| Samantha Ferris | as | Leslie Handleman (voice) (as Samantha Farris) |
| Mark Oliver | as | Markus Van Der Hellen (voice) |
| Eva De Viveiros | as | Clair Thomas/Madame Lazora (voice) (as Eva DeViverios) |
