| Cast list |
| J. Grant Albrecht | as | Unit Response Voice (voice) (as Grant Albrecht) |
| Maurizio Barra | as | Fremen #1 |
| Michael Bell | as | Unit Response Voice (voice) |
| Gregg Berger | as | Executrix Council (voice) |
| Rick Blakely | as | Unit Reponse Voice (voice) |
| Eugenie Bondurant | as | Executrix Speaker |
| Mark Bringleson | as | Shafla (as Mark Bringelson) |
| Lucy Butler | as | Reverend Mother |
| Hamilton Camp | as | Unit Reponse Voice (voice) |
| Lee Cherry | as | Unit Reponse Voice (voice) |
| Joel Christian | as | Gunseng Harkonnen |
| Alexander Colom | as | Tortured Man, Guard |
| Gary Cox | as | Guard #2 |
| Esteban Cueto | as | Fremen #2 (as Estaban Cueto) |
| Shane Dietrich | as | Guard #3 |
| Brian Dilley | as | Guard #4 |
| Sean Donnellan | as | Unit Reponse Voice (voice) |
| Michael Dorn | as | Duke Achillus |
| Daniel Etter | as | Guard #1 |
| Quinton Flynn | as | Unit Reponse Voice (voice) |
| Ann Galati | as | Executrix Council |
| Benjamin Galley | as | Guard #5 |
| Rich Green | as | Unit Reponse Voice (voice) (as Richard Green) |
| Matthew Hansel | as | Guard #6 |
| Beau Hopkins | as | Guard #7 |
| Anastasia Horne | as | Sardaukar Servant |
| Nick Jameson | as | Unit Reponse Voice (voice) |
| John Jett | as | Guard #8 |
| Igor Jijikine | as | Fremen #3 (as Igor Jijenke) |
| Daniel Kucan | as | Geisel |
| Kay E. Kuter | as | Unit Reponse Voice (voice) |
| Chris Longpre | as | Guard #9 |
| Levi Luke | as | Ordos Scientist |
| Stefan Marks | as | Unit Reponse Voice (voice) |
| Vanessa Marshall | as | Filmbook, Unit Reponse Voice (voice) |
| Michael McShane | as | Baron Harkonnen |
| Rhino Michaels | as | Caid |
| Diane Michelle | as | Executrix Council (voice) |
| Iona Morris | as | UI Computer (voice) |
| Ted Morris | as | Guard #10 (as Theodore A. Morris) |
| Minae Noji | as | Roma Atani |
| Jack Orend | as | Cilix (as Jack R. Orend) |
| Billy O'Sullivan | as | Unit Reponse Voice (voice) |
| Bryan Philibald | as | Ordos Scientist |
| David Reynolds | as | Copec Harkonnen |
| Ben Robertson | as | Fremen #4 |
| Neil Ross | as | Executrix Council, Unit Reponse Voice (voice) |
| Michael Ruppert | as | Guard #11 |
| Jody Sadler | as | Harkonnen Servant |
| Steven I. Schafer | as | Severed Head (as Steven Ira Schafer) |
| Vincent Schiavelli | as | Yanich Kobal |
| Heart Sharpe | as | Executrix Council |
| Susan Silo | as | Executrix Council (voice) |
| Joseph Smith | as | Executrix Council |
| Michael Bailey Smith | as | Burseg |
| Jonny Solomon | as | Unit Reponse Voice (voice) (as Johnny Solomon) |
| Adrian Sparks | as | Emperor Corrino |
| Tom Szakolczay | as | Guard #12 |
| Philip Tanzini | as | Unit Reponse Voice (voice) (as Phil Tanzini) |
| Rudolph Thail | as | Executrix Council |
| Scott Vance | as | Corlen |
| Musetta Vander | as | Lady Elara |
| Gary Velasco | as | Fremen #5 |
| Ryan Vervack | as | Facedancer Assassin |
| Gregory Voyticek | as | Fremen #6 |
| Doug Wilson | as | Guard #13 |
| Nicholas Worth | as | Kolinar Koltrass |
