| Cast list |
| William Shatner | as | Captain James T. Kirk (voice) |
| Patrick Stewart | as | Captain Jean Luc Picard (voice) |
| Avery Brooks | as | Captain Benjamin Sisko (voice) |
| Kate Mulgrew | as | Admiral Kathryn Janeway (voice) |
| Scott Bakula | as | Captain Jonathan Archer (voice) |
| Susan Allenback | as | Various crew (voice) |
| Jeff Baker | as | Captain Radonovich, USS Scott Captain, Romulan, Borg |
| Bari Biern | as | T'Uerell |
| Jonathan Bryce | as | Captain Jameson / ENT Captain / TOS crew / Klingon |
| Linda Canyon | as | Medical Ship First Officer / TOS Captain / Romulan / Borg (as Linda Kenyon) |
| David DeBoy | as | Reggie / TOS Captain / Romulan |
| Shari Elliker | as | Sub-Commander Vorel / Romulan / TNG Captain / Medical Ship Crew / Starbase 61 crew / TOS Crew |
| Ty Ford | as | Commander Lo'Tal / Romulan / Klingon / ENT Captain / Borg / TOS crew |
| David Jackson | as | Ruhalter / Lt. Garrison / Borg / Romulan |
| Wes Johnson | as | Menvek / Klingon Captain / Borg / TOS Captain / TNG Crew / TOS Helm |
| Kathryn Klvana | as | Research Station Away Team / ENT crew / Borg / Medical Ship crew |
| Mike Rosson | as | Tuc'Nev / Klingon / Borg / TNG crew / ENT crew |
| Craig Sechler | as | Captain Hollister / ENT crew / Klingon / Romulan |
