| Cast list |
| Scott Innes | as | Scooby-Doo /Shaggy (voice) |
| Frank Welker | as | Fred / Prof. Stoker (voice) |
| Grey DeLisle | as | Daphne Blake / Marcy (voice) |
| Mindy Cohn | as | Velma (voice) |
| Adam West | as | Winslow Stanton (voice) |
| Susanne Blakeslee | as | Dame Nella Vivante (voice) |
| Kim Mai Guest | as | Nikki / Maggie Xi / Kung Fu Maiden / Fire-Breathing Groupie (voice) |
| Chris Edgerly | as | Guitar Ghoul / Alvin Wiener / Jed / Juggling Clown / Ninja / Pterodactyl / 10000 Volt Ghost (voice) |
| Fred Tatasciore | as | Joe Grimm / Zen Tuo / Ho Fong / Circus Strongman / Black Knight / Caveman / Chinese Zombie (voice) |
